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OCT4 抗体 (N-Term)

The 兔 多克隆 anti-OCT4 antibody (ABIN127203) specifically detects OCT4 in WB, IP 和 IHC (p). The antibody is reactive with 人, 小鼠, 大鼠, Cow 和 Pig samples.
产品编号 ABIN127203
发货至: 中国
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Quick Overview for OCT4 抗体 (N-Term) (ABIN127203)

抗原

See all OCT4 (POU5F1) 抗体
OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

适用

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人, 小鼠, 大鼠, Cow, Pig

宿主

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克隆类型

  • 100
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多克隆

标记

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This OCT4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    N-Term

    特异性

    The antibody reacts with 45 kDa Oct-4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B

    纯化方法

    Affinity Chromatography.

    免疫原

    Synthetic peptide mapping to the N-terminus of mouse Oct-4.
  • 应用备注

    Western blot: 0.5-4 μg/mL. Immunoprecipitation: 20 μg/mL. Immunohistochemistry: 20 μg/mL. Positive Control: Rat kidney tissue lysate.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    PBS, pH 7.2, 0.01 % Thimerosal, 30 % Glycerol, 0.5 % BSA

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C/-80 °C

    储存方法

    Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for longer.
  • 抗原

    OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

    别名

    OCT3/4

    背景

    Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1

    基因ID

    5460

    NCBI登录号

    NP_002692

    UniProt

    Q01860

    途径

    Stem Cell Maintenance
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