OCT4 抗体 (N-Term)
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Quick Overview for OCT4 抗体 (N-Term) (ABIN127203)
抗原
See all OCT4 (POU5F1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- The antibody reacts with 45 kDa Oct-4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B
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纯化方法
- Affinity Chromatography.
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免疫原
- Synthetic peptide mapping to the N-terminus of mouse Oct-4.
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应用备注
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Western blot: 0.5-4 μg/mL. Immunoprecipitation: 20 μg/mL. Immunohistochemistry: 20 μg/mL. Positive Control: Rat kidney tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- PBS, pH 7.2, 0.01 % Thimerosal, 30 % Glycerol, 0.5 % BSA
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C/-80 °C
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储存方法
- Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for longer.
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- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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别名
- OCT3/4
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背景
- Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
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基因ID
- 5460
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NCBI登录号
- NP_002692
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UniProt
- Q01860
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途径
- Stem Cell Maintenance
抗原
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