Caspase 3 抗体 (active, Cleavage Site, N-Term)
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Quick Overview for Caspase 3 抗体 (active, Cleavage Site, N-Term) (ABIN126957)
抗原
See all Caspase 3 (CASP3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- active, Cleavage Site, N-Term
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特异性
- The antibody preferentially recognizes the p17 fragment of the active caspase-3.
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纯化方法
- Affinity chromatography
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免疫原
- Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
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应用备注
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Western Blot: 0.5-4 μg/mL. Immunohistochemistry on paraffin sections: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C/-80 °C
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储存方法
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.
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- Caspase 3 (CASP3)
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别名
- Caspase-3
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背景
- Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.Synonyms: Apopain, CASP-3, CASP3, CPP-32, CPP32, Cysteine protease CPP32, SCA-1, SCA1, SREBP cleavage activity 1, Yama protein
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基因ID
- 836
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NCBI登录号
- NP_004337
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UniProt
- P42574
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
抗原
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