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Vimentin 抗体

The 小鼠 单克隆 anti-Vimentin antibody is suitable to detect Vimentin in samples from 人, 小鼠 和 犬. It has been validated for WB.
产品编号 ABIN126914
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Vimentin 抗体 (ABIN126914)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, 小鼠, 犬

宿主

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小鼠

克隆类型

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单克隆

标记

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This Vimentin antibody is un-conjugated

应用范围

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Western Blotting (WB)

克隆位点

11H6
  • 特异性

    This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.

    纯化方法

    Size exclusion chromatography

    组件

    incl. positive Control

    免疫原

    Synthetic peptide conjugated to hemocyanin

    亚型

    IgG
  • 应用备注

    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore with 1 mL H2O (15 min, RT).

    缓冲液

    1 mL2 x PBS containing 0.09 % Sodium Azide/PEG and Sucrose.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C/-80 °C

    储存方法

    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    背景

    Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.Synonyms: VIM

    分子量

    54 kDa

    基因ID

    7431

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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