Vimentin 抗体
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北京 101111
Quick Overview for Vimentin 抗体 (ABIN126914)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.
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纯化方法
- Size exclusion chromatography
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组件
- incl. positive Control
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免疫原
- Synthetic peptide conjugated to hemocyanin
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亚型
- IgG
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2 x PBS containing 0.09 % Sodium Azide/PEG and Sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Vimentin (VIM)
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别名
- Vimentin
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背景
- Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.Synonyms: VIM
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分子量
- 54 kDa
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基因ID
- 7431
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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