Topoisomerase I 抗体
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北京 101111
Quick Overview for Topoisomerase I 抗体 (ABIN126904)
抗原
See all Topoisomerase I (TOP1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody specifically recognizes Topoisomerase 1 in Western blot applications.
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产品特性
- Synonyms: Top-1, DNA topoisomerase 1, DNA topoisomerase I
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纯化方法
- Size Exclusion Chromatography
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免疫原
- Synthetic peptide conjugated to KLH
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亚型
- IgG1
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anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibody
TOP1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人 WB, ELISA, IHC, IP, FACS 宿主: 兔 Monoclonal 6D8 unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I (TOP1) (Internal Region) antibodyTOP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 1-100) antibodyTOP1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (N-Term) antibodyTOP1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 1291CT875-142-166 unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 715-765) antibodyTOP1 适用: 人, 小鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB7195 unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB2060 unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I (TOP1) (AA 2-31), (N-Term) antibodyTOP1 适用: 人 WB, IF 宿主: 兔 Polyclonal RB34034 unconjugated
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detectionRecommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
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背景
- Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100 kDa protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then relegated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus, and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability. DNA Topoisomerases catalyze strand breakage of DNA molecules. During DNA cleavage, a tyrosyl residue of the enzyme breaks the DNA strand by forming a covalent phosphotyrosine residue. Rejoining of the DNA strands occurs by a second transesterification reaction.Synonyms: DNA topoisomerase 1, DNA topoisomerase I, Top-1
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分子量
- 90 kDa
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基因ID
- 7150
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UniProt
- P11387
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途径
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
抗原
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