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- 抗原 See all PLSCR1 抗体
- PLSCR1 (phospholipid Scramblase 1 (PLSCR1))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PLSCR1 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa.
- 产品特性
- Synonyms: Phospholipid scramblase 1, PL scramblase 1, MmTRA1b, Ca(2+)-dependent phospholipidscramblase 1, Erythrocyte phospholipid scramblase
- 纯化方法
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
- 组件
- incl. positive Control
- 免疫原
- Synthetic peptide conjugated to KLH.
- 克隆位点
- 1-00E-009
- 亚型
- IgG1
- Top Product
- Discover our top product PLSCR1 Primary Antibody
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anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibody
PLSCR1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) antibodyPLSCR1 适用: 大鼠 WB, IP, FACS, ICC 宿主: 小鼠 Monoclonal 13A6 [TEC-23] unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) antibodyPLSCR1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) antibodyPLSCR1 适用: 大鼠 IP, IF, FACS, ICC 宿主: 小鼠 Monoclonal 13A6 [TEC-23] unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1F9 unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 ELISA, IP 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (N-Term) antibodyPLSCR1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Positive Control Cell LysateHepG2 untreatedSize Sufficient: 20 mini-gel lanes. Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for Immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT).
- 缓冲液
- 1 mLPBS containing 0.09 % Sodium Azide as preservative, PEG and Sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- PLSCR1 (phospholipid Scramblase 1 (PLSCR1))
- Abstract
- PLSCR1 产品
- 别名
- mmtra1b antibody, MGC84969 antibody, PLSCR1 antibody, MGC148322 antibody, MMTRA1B antibody, MmTRA1a antibody, MmTRA1b antibody, Nor1 antibody, Tra1 antibody, Tra1a antibody, Tra1b antibody, Tras1 antibody, Tras2 antibody, PLSCR2 antibody, phospholipid scramblase 1 L homeolog antibody, phospholipid scramblase 1 antibody, plscr1.L antibody, PLS1 antibody, PVX_111580 antibody, PLSCR1 antibody, plscr1 antibody, Plscr1 antibody, LOC100712949 antibody, PLSCR2 antibody, LOC100341366 antibody, LOC611500 antibody
- 背景
- Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases.Synonyms: Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1, Phospholipid scramblase 1
- 基因ID
- 5359
- UniProt
- O15162
- 途径
- Cellular Response to Molecule of Bacterial Origin
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