PLSCR1 抗体 (N-Term)
Quick Overview for PLSCR1 抗体 (N-Term) (ABIN126886)
抗原
See all PLSCR1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa.
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产品特性
- Synonyms: Phospholipid scramblase 1, PL scramblase 1, MmTRA1b, Ca(2+)-dependent phospholipidscramblase 1, Erythrocyte phospholipid scramblase
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纯化方法
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
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组件
- incl. positive Control
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免疫原
- Synthetic peptide conjugated to KLH.
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亚型
- IgG1
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anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibody
PLSCR1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) antibodyPLSCR1 适用: 大鼠 WB, IP, FACS, ICC 宿主: 小鼠 Monoclonal 13A6 [TEC-23] unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) antibodyPLSCR1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) antibodyPLSCR1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 ELISA, IP 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (AA 1-318) antibodyPLSCR1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1F9 unconjugated
anti-phospholipid Scramblase 1 (PLSCR1) (N-Term) antibodyPLSCR1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control Cell LysateHepG2 untreatedSize Sufficient: 20 mini-gel lanes. Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for Immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
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限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mLPBS containing 0.09 % Sodium Azide as preservative, PEG and Sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- PLSCR1 (phospholipid Scramblase 1 (PLSCR1))
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背景
- Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases.Synonyms: Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1, Phospholipid scramblase 1
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基因ID
- 5359
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UniProt
- O15162
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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