This antibody specifically interacts with activated Pyk2/CAKβ phosphorylated at tyrosine 402. The antibody does not crossreact with the non-phosphorylated form of Pyk2/CAKβ nor with unrelated phosphorylation sites.
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1 mL H2O (15 min, RT).
缓冲液
1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C/-80 °C
储存方法
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Shelf life: one year from despatch.
有效期
12 months
抗原
PTK2B
(PTK2B Protein tyrosine Kinase 2 beta (PTK2B))
Proline-rich tyrosine kinase 2 (Pyk2, also known as cellular adhesion kinase β, CAK β) is a 116 kDa cytoplasmic tyrosine kinase. Pyk2 is rapidely phosphorylated on tyrosine residues in response to activation by G-protein-coupled receptors as well as stress signals. Phosphorylation of Pyk2 leads to activation of stress-activated kinase 1 and 2 (SAPK1 and SAPK2).Synonyms: CADTK, CAK-beta, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, Focal adhesion kinase 2, PYK2, Proline-rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, RAFTK, Related adhesion focal tyrosine kinase