Nitrotyrosine 抗体
Quick Overview for Nitrotyrosine 抗体 (ABIN126845)
抗原
See all Nitrotyrosine 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
产品特性
- Synonyms: NO-Tyrosine, Nitro-Tyrosine
-
纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography
-
免疫原
- KLH modified with Nitrotyrosine
-
亚型
- IgG1
-
-
anti-Nitrotyrosine antibody
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 unconjugated
anti-Nitrotyrosine antibody (HRP)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 HRP
anti-Nitrotyrosine antibody (FITC)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 FITC
anti-Nitrotyrosine antibody (Biotin)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Biotin
anti-Nitrotyrosine antibody (PE)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PE
anti-Nitrotyrosine antibody (APC)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 APC
anti-Nitrotyrosine antibody (PerCP)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PerCP
anti-Nitrotyrosine antibody (Atto 488)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 488
anti-Nitrotyrosine antibody (Atto 594)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 594
anti-Nitrotyrosine antibody (Atto 390)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 390
-
-
应用备注
-
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with 1 mL H2O (15 min, RT)
-
缓冲液
- 1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C/-80 °C
-
储存方法
-
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
-
-
- Nitrotyrosine
-
物质类
- Chemical
-
背景
- The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.Synonyms: NO-Tyrosine, Nitro-Tyrosine
抗原
-
