ENOS 抗体 (pSer1177)
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北京 101111
Quick Overview for ENOS 抗体 (pSer1177) (ABIN126844)
抗原
See all ENOS (NOS3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer1177
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特异性
- This antibody specifically recognizes eNOS phosphorylated at Serine 1177.
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产品特性
- Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS, Nitric oxide synthase,endothelial
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纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography
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免疫原
- Synthetic phosphopeptide conjugated to KLH
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亚型
- IgG1
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from pervanadate treated Bend3 cells. ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide, 50 % Glycerol, PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
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别名
- NOS3
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背景
- Endothelial NO synthase (eNOS) produces nitric oxid (NO) which plays a crucial role in cardiovascular homeostasis. Activation of the enzyme and NO production can be induced by phosphorylation of eNOS at serine 1177 by activated Akt/PKB.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial
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基因ID
- 4846
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UniProt
- P29474
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途径
- ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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