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c-FOS 抗体 (N-Term)

This anti-c-FOS antibody is a 小鼠 单克隆 antibody detecting c-FOS in WB, IHC (fro) 和 EIA. Suitable for 人, 大鼠, 小鼠 和 犬.
产品编号 ABIN126801
发货至: 中国

Quick Overview for c-FOS 抗体 (N-Term) (ABIN126801)

抗原

See all c-FOS (c-Fos) 抗体
c-FOS (c-Fos) (c-Fos)

适用

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人, 大鼠, 小鼠, 犬

宿主

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小鼠

克隆类型

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单克隆

标记

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This c-FOS antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

克隆位点

8B5
  • 抗原表位

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    N-Term

    特异性

    This antibody specifically interacts with the N-terminus of c-Fos. The antibody is suitable for Western blot and ELISA applications. It can be used for the detection of cellular Fos levels that might dramatically change during signal transduction. It is an important tool in combination with mab Fos-34E4 (phospho-Ser374) for studying fos expression and phosphorylation.

    产品特性

    Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7

    纯化方法

    Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

    组件

    incl. positive Control

    免疫原

    Synthetic peptide conjugated to KLH.

    亚型

    IgG1
  • 应用备注

    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore with 1 mL H2O (15 min, RT).

    缓冲液

    1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C/-80 °C

    储存方法

    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch

    有效期

    12 months
  • 抗原

    c-FOS (c-Fos) (c-Fos)

    别名

    C-Fos

    背景

    The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos

    基因ID

    2353

    UniProt

    P01100

    途径

    S100 Proteins
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