c-FOS 抗体 (N-Term)
Quick Overview for c-FOS 抗体 (N-Term) (ABIN126801)
抗原
See all c-FOS (c-Fos) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- N-Term
-
特异性
- This antibody specifically interacts with the N-terminus of c-Fos. The antibody is suitable for Western blot and ELISA applications. It can be used for the detection of cellular Fos levels that might dramatically change during signal transduction. It is an important tool in combination with mab Fos-34E4 (phospho-Ser374) for studying fos expression and phosphorylation.
-
产品特性
- Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7
-
纯化方法
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
-
组件
- incl. positive Control
-
免疫原
- Synthetic peptide conjugated to KLH.
-
亚型
- IgG1
-
-
-
-
应用备注
-
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with 1 mL H2O (15 min, RT).
-
缓冲液
- 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C/-80 °C
-
储存方法
-
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch -
有效期
- 12 months
-
-
- c-FOS (c-Fos) (c-Fos)
-
别名
- C-Fos
-
背景
- The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos
-
基因ID
- 2353
-
UniProt
- P01100
-
途径
- S100 Proteins
抗原
-