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c-FOS 抗体 (pSer374)

This anti-c-FOS antibody is a 小鼠 单克隆 antibody detecting c-FOS in WB, IHC (fro) 和 EIA. Suitable for 人 和 犬.
产品编号 ABIN126800
发货至: 中国

Quick Overview for c-FOS 抗体 (pSer374) (ABIN126800)

抗原

See all c-FOS (c-Fos) 抗体
c-FOS (c-Fos) (c-Fos)

适用

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人, 犬

宿主

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小鼠

克隆类型

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单克隆

标记

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This c-FOS antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

克隆位点

3-40E-005
  • 抗原表位

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    pSer374

    特异性

    This antibody specifically interacts with c-Fos phosphorylated at serine 374.

    产品特性

    Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7

    纯化方法

    Size exclusion chromatography

    组件

    incl. positive Control

    免疫原

    Synthetic phosphopeptide conjugated to KLH.

    亚型

    IgG1
  • 应用备注

    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore with 1 mL H2O (15 min, RT).

    缓冲液

    1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C/-80 °C

    储存方法

    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch

    有效期

    12 months
  • 抗原

    c-FOS (c-Fos) (c-Fos)

    别名

    C-Fos

    背景

    The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos

    基因ID

    2353

    UniProt

    P01100

    途径

    S100 Proteins
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