c-FOS 抗体 (pSer374)
Quick Overview for c-FOS 抗体 (pSer374) (ABIN126800)
抗原
See all c-FOS (c-Fos) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer374
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特异性
- This antibody specifically interacts with c-Fos phosphorylated at serine 374.
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产品特性
- Synonyms: FOS, G0S7, Proto-oncogene protein c-fos, Cellular oncogene fos, G0/G1 switch regulatoryprotein 7
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纯化方法
- Size exclusion chromatography
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组件
- incl. positive Control
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免疫原
- Synthetic phosphopeptide conjugated to KLH.
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亚型
- IgG1
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch -
有效期
- 12 months
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- c-FOS (c-Fos) (c-Fos)
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别名
- C-Fos
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背景
- The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.Synonyms: Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos
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基因ID
- 2353
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UniProt
- P01100
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途径
- S100 Proteins
抗原
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