CREB1 抗体 (pSer133)
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北京 101111
Quick Overview for CREB1 抗体 (pSer133) (ABIN126754)
抗原
See all CREB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer133
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特异性
- This antibody specifically recognizes CREB phosphorylated at serine 133 and ATF1 phosphorylated at serine 63 in Western blot and ELISA applications.
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产品特性
- Synonyms: cAMP Response Element-Binding protein, CREB1, CREB-1, Cyclic AMP-dependenttranscription factor ATF1
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纯化方法
- Size exclusion chromatography
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组件
- incl. positive Control
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免疫原
- Synthetic phosphopeptide conjugated to KLH.
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亚型
- IgG1
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
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别名
- CREB1
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背景
- The transcription factor CREB activates transcription in response to a variety of extracellular signals. Various kinases, including Protein kinase A (PKA) , and CaM kinase II, regulate CREB activity by phosphorylation of serine 133. Molecular Weight: CREB:43 kDa, ATF1:38 kDaSynonyms: CREB-1, CREB1, Cyclic AMP-dependent transcription factor ATF1, cAMP Response Element-Binding protein
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基因ID
- 1385
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UniProt
- P16220
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途径
- TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
抗原
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