CDH2
适用: 人
WB, IHC, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for moredetails). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
Positive Control: Cell lysate from untreated SH-SY5Y cellsFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells.
限制
仅限研究用
溶解方式
Restore with 1 mL H2O (15 min, RT).
缓冲液
1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C/-80 °C
储存方法
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin.Synonyms: CDH2, CDHN, CDw325, Cadherin N, N-Cadherin, NCAD, Neural cadherin