LYVE1 抗体 (AA 24-228) (Biotin)
Quick Overview for LYVE1 抗体 (AA 24-228) (Biotin) (ABIN126137)
抗原
See all LYVE1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 24-228
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特异性
- Highly specifc for Mouse Lyve-1.
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交叉反应 (详细)
- Species reactivity (tested):Mouse.
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纯化方法
- Antigen Affinity Chromatography
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免疫原
- Highly pure recombinant Mouse soluble LYVE-1 produced in insect cells (Cat.-No DA3524). Remarks: The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).
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应用备注
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The unconjugated antibody can be used for ELISA (1-15 μg/mL), Western blotting (1-2 μg/mLwith the appropriate secondary reagents), FACS (3-20 μg/mL together with the appropriatesecondary reagents) and Cell sorting. The antibody is also suitable for Immunohistochemistry on on Paraffin as well as on FrozenSections (0.25-4 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with sterile water to a concentration of 0.1-1.0 μg/mL. Centrifuge vial prior to opening.
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缓冲液
- PBS, pH 7.4, 0.02 % Sodium Azide, 50x BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))
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别名
- LYVE-1
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背景
- LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 Molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 Molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.Synonyms: CRSBP-1, CRSBP1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, HAR, Hyaluronic acid receptor, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, XLKD1
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基因ID
- 114332
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NCBI登录号
- NP_444477
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UniProt
- Q8BHC0
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途径
- Glycosaminoglycan Metabolic Process
抗原
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