KRT20 抗体
Quick Overview for KRT20 抗体 (ABIN125990)
抗原
See all KRT20 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reacts with human, pig, not with rat. Reacts with HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
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纯化方法
- Protein A affinity chromatography
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免疫原
- Electrophoretically purified keratin K20 from human intestinal mucosa.
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亚型
- IgG2a
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anti-Keratin 20 (KRT20) antibody
KRT20 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 1-424) antibodyKRT20 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2G3-1C8 unconjugated
anti-Keratin 20 (KRT20) (AA 245-424) antibodyKRT20 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal KRT20-1992 unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal KRT20-1991 unconjugated
anti-Keratin 20 (KRT20) (AA 1-266) antibodyKRT20 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4A2 unconjugated
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal KRT20-3129R unconjugated Recombinant Antibody
anti-Keratin 20 (KRT20) (AA 196-323) antibodyKRT20 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal KRT20-3145 unconjugated
anti-Keratin 20 (KRT20) (AA 1-150) antibodyKRT20 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C7B9 unconjugated
anti-Keratin 20 (KRT20) antibodyKRT20 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 1F10 unconjugated
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应用备注
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Immunohistochemical on frozen sections: 1: 10, 1h at RT or over night at 2-8C. Immunohistochemical on cytological material. Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in 1 mL dist. water
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缓冲液
- PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- This product is photosensitive and should be protected from light
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储存条件
- 4 °C
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储存方法
- Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- KRT20 (Keratin 20 (KRT20))
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别名
- Cytokeratin 20
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背景
- Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
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基因ID
- 54474
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NCBI登录号
- NP_061883
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UniProt
- P35900
抗原
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