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Cytokeratin 7 抗体

KRT7 适用: 人, Cow, Pig, 绵羊 WB, IF, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal Ks 7-18 unconjugated
产品编号 ABIN125985
发货至: 中国
  • 抗原 See all Cytokeratin 7 (KRT7) 抗体
    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
    适用
    • 204
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    人, Cow, Pig, 绵羊
    宿主
    • 120
    • 93
    小鼠
    克隆类型
    • 120
    • 92
    • 1
    单克隆
    标记
    • 91
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    This Cytokeratin 7 antibody is un-conjugated
    应用范围
    • 130
    • 88
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    This antibody represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative. Detects specific subtypes of adenocarcinomas: Adenocarcimona of pancreas, gallbladder, lung, cervix, cholangio carcinoma of liver, ductal and lobular carcinoma of breast, carcinomas of ovary, transitional cell carcinoma of bladder, mesothelioma, negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed. Reacts with Mr 54,000 polypeptide (keratin K7, formerly also designated Cytokeratin 7) of human glandular epithelia.
    纯化方法
    Affinity Chromatography on Protein A .
    免疫原
    Cytoskeletal proteins from cultured HeLa cells.
    克隆位点
    Ks 7-18
    亚型
    IgG1
    Top Product
    Discover our top product KRT7 Primary Antibody
  • 应用备注
    Immunohistochemistry on Paraffin Sections: 1/50 (Overnight at 2-8 °C). Protease pretreatment is recommended prior to antibody application. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT). Cytological Material. Immunoblotting.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in 1 mL distilled water
    缓冲液
    Final Solution contains PBS buffer, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    This product is photosensitive and should be protected from light
    储存条件
    4 °C
    储存方法
    Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • 抗原
    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
    别名
    Cytokeratin 7 (KRT7 产品)
    别名
    CK7 antibody, K2C7 antibody, K7 antibody, SCL antibody, D15Wsu77e antibody, Krt2-7 antibody, KRT7 antibody, keratin 7 antibody, KRT7 antibody, Krt7 antibody
    背景
    Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin
    基因ID
    3855
    NCBI登录号
    NP_005547
    UniProt
    P08729
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