(3 references)-
- 抗原 See all CD5 抗体
- CD5
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD5 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
- 交叉反应 （详细）
- Human, Other species Not tested
- 纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- stimulated human leukocytes
- 克隆位点
- CRIS1
- 亚型
- IgG2a
- Top Product
- Discover our top product CD5 Primary Antibody
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anti-CD5 (CD5) (AA 105-210) antibody
CD5 适用: 人 WB 宿主: 山羊 Polyclonal unconjugated
anti-CD5 (CD5) (AA 269-366) antibodyCD5 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal CD5-2416 unconjugated
anti-CD5 (CD5) (AA 25-372) antibodyCD5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD5 (CD5) (AA 269-366) antibodyCD5 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal CD5-2418 unconjugated
anti-CD5 (CD5) antibodyCD5 适用: 人 FACS, WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal L17F12 unconjugated
anti-CD5 (CD5) (AA 27-233) antibodyCD5 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 6A11 unconjugated
anti-CD5 (CD5) (AA 403-495) antibodyCD5 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6C3B2 unconjugated
anti-CD5 (CD5) (AA 403-495) antibodyCD5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F7 unconjugated
anti-CD5 (CD5) antibodyCD5 适用: 人 FACS, WB, IP, ELISA, IHC (fro) 宿主: 小鼠 Monoclonal CRIS1 unconjugated
anti-CD5 (CD5) antibodyCD5 适用: 人 FACS, WB, IP, ELISA, IHC (p) 宿主: 小鼠 Monoclonal MEM-32 unconjugated
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- 应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
- 说明
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- 限制
- 仅限研究用
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- 溶解方式
- No reconstitution is necessary.
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Conformation of the hypervariable region L3 without the key proline residue." in: Protein science : a publication of the Protein Society, Vol. 5, Issue 1, pp. 167-9, (1996) (PubMed).
: "Intracellular events involved in CD5-induced human T cell activation and proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 5, pp. 1287-93, (1992) (PubMed).
: "Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis." in: Nephron, Vol. 53, Issue 1, pp. 41-9, (1989) (PubMed).
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: "Conformation of the hypervariable region L3 without the key proline residue." in: Protein science : a publication of the Protein Society, Vol. 5, Issue 1, pp. 167-9, (1996) (PubMed).
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- 抗原
- CD5
- 别名
- CD5 (CD5 产品)
- 别名
- CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody
- 背景
- CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1
- 基因ID
- 921
- UniProt
- P06127
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