LIME 抗体 (Intracellular)
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Quick Overview for LIME 抗体 (Intracellular) (ABIN120743)
抗原
See all LIME (LIME1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 141-295, Intracellular
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特异性
- The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.
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纯化方法
- Protein A affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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免疫原
- Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME
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亚型
- IgG1
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anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibody
LIME1 适用: 人 IP, ICFC 宿主: 小鼠 Monoclonal LIME-06 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 170-295) antibodyLIME1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibodyLIME1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 281-296) antibodyLIME1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal LIME-10 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (C-Term) antibodyVerified LIME1 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 86-202) antibodyLIME1 适用: 小鼠 WB, IP 宿主: 小鼠 Monoclonal mLIME-05 unconjugated
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应用备注
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Suitable for Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 containing 15 mM sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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- LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
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别名
- LIME1
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背景
- LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1
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基因ID
- 54923
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UniProt
- Q9H400
抗原
-
