ACRV1 抗体
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北京 101111
Quick Overview for ACRV1 抗体 (ABIN120713)
抗原
See all ACRV1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody reacts with intra-acrosomal sperm proteins (220, 50, 34 and 21 kDa). Reacts with Human, Porcine (Pig).
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产品特性
- Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
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纯化方法
- Precipitation methods and size-exclusion chromatography
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.
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亚型
- IgM
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anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibody
ACRV1 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5E3D6 unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2D10D5 unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 1-265) antibodyACRV1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人, 豚鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) antibodyACRV1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunocytochemistry: 10 μg/mL, membrane permeabilization (acetone) essential. Flow Cytometry. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
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别名
- ACRV1
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背景
- One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2
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基因ID
- 56
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UniProt
- P26436
抗原
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