ACRV1 抗体
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- 抗原 See all ACRV1 抗体
- ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
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适用
- 人, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ACRV1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
- 特异性
- The antibody reacts with intra-acrosomal sperm proteins (220, 50, 34 and 21 kDa). Reacts with Human, Porcine (Pig).
- 产品特性
- Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
- 纯化方法
- Precipitation methods and size-exclusion chromatography
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.
- 克隆位点
- Hs-8
- 亚型
- IgM
- Top Product
- Discover our top product ACRV1 Primary Antibody
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anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibody
ACRV1 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5E3D6 unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2D10D5 unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 22-265) antibodyACRV1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人, 豚鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (AA 1-265) antibodyACRV1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) antibodyACRV1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Acrosomal Vesicle Protein 1 (ACRV1) (N-Term) antibodyACRV1 适用: 人, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunocytochemistry: 10 μg/mL, membrane permeabilization (acetone) essential. Flow Cytometry. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
- 别名
- ACRV1 (ACRV1 产品)
- 背景
- One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2
- 基因ID
- 56
- UniProt
- P26436
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