The antibody reacts with intra-acrosomal proteins (92 and 42 kDa) of sperms.
产品特性
Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
纯化方法
Protein-A affinity chromatography
纯度
> 95 % pure (by SDS-PAGE)
免疫原
Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10%glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% acetic acid,lyophylized and subsequently used for immunization.
Immunocytochemistry, membrane permeabilization (acetone) essential. Western Blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Canine intra-acrosomal sperm protein (92 and 42 kDa) is a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2