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Cytokeratin 7 抗体

KRT7 适用: 人, Cow WB, IF, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal C-46 unconjugated
产品编号 ABIN120627
发货至: 中国
  • 抗原 See all Cytokeratin 7 (KRT7) 抗体
    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
    适用
    • 205
    • 69
    • 58
    • 6
    • 6
    • 4
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    • 3
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    • 2
    • 1
    • 1
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    人, Cow
    宿主
    • 120
    • 94
    小鼠
    克隆类型
    • 121
    • 92
    • 1
    单克隆
    标记
    • 92
    • 21
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    • 2
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    This Cytokeratin 7 antibody is un-conjugated
    应用范围
    • 131
    • 88
    • 86
    • 86
    • 43
    • 34
    • 27
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    • 19
    • 15
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa).
    交叉反应 (详细)
    Species reactivity (tested):Human, Porcine, Bovine.
    Also reacts to: KRT17
    产品特性
    Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin, Keratin type II cytoskeletal 7
    纯化方法
    Protein A-affinity chromatography
    纯度
    > 95 % by SDS-PAGE
    免疫原
    Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
    克隆位点
    C-46
    亚型
    IgG1
    Top Product
    Discover our top product KRT7 Primary Antibody
  • 应用备注
    Immunoprecipitation. Immunocytochemistry. Western Blot: 2-3 mg/mL, 60 min in RT, Boil for 3 min, Resuspend dry pelet in solubilizelysis buffer (with carbamide and detergent NP-40), Mix lysate with reducing SDS-PAGE (+merkaptoethanol) sample buffer, Reducing conditions. Positive control: HeLa human cell line. Immunohistochemistry on paraffin sections: 20 mg/mL, 0,1 % pepsin, 30 min, 37C, 4Covernight, chromogen DAB. Positive tissue: pancreas.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, pH ~7.4, 15 mM Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
    别名
    Cytokeratin 7 (KRT7 产品)
    别名
    CK7 antibody, K2C7 antibody, K7 antibody, SCL antibody, D15Wsu77e antibody, Krt2-7 antibody, KRT7 antibody, keratin 7 antibody, KRT7 antibody, Krt7 antibody
    背景
    Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin
    基因ID
    3855
    UniProt
    P08729
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