Cytokeratin 7 抗体
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北京 101111
Quick Overview for Cytokeratin 7 抗体 (ABIN120627)
抗原
See all Cytokeratin 7 (KRT7) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa).
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交叉反应 （详细）
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Species reactivity (tested):Human, Porcine, Bovine.
Also reacts to: KRT17 -
产品特性
- Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin, Keratin type II cytoskeletal 7
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纯化方法
- Protein A-affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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免疫原
- Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
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亚型
- IgG1
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anti-Keratin 7 (KRT7) (AA 251-350) antibody
KRT7 适用: 人, 小鼠 WB, FACS, IHC (p), ELISA, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 91-394) antibodyKRT7 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 394-457) antibodyKRT7 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS 宿主: 小鼠 Monoclonal rOV-TL12-30 unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) (AA 2-469) antibodyKRT7 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠 IHC, IF, FACS 宿主: 小鼠 Monoclonal KRT7-760-OV-TL12-30 unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS 宿主: 兔 Monoclonal KRT7-1499R unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 IHC, IF, FACS 宿主: 小鼠 Monoclonal KRT7-903 unconjugated
anti-Keratin 7 (KRT7) (C-Term) antibodyKRT7 适用: 人 WB, IHC, FACS, ICC, IP 宿主: 兔 Monoclonal BC1 unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) antibodyKD Validated KRT7 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB2830 unconjugated Recombinant Antibody
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应用备注
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Immunoprecipitation. Immunocytochemistry. Western Blot: 2-3 mg/mL, 60 min in RT, Boil for 3 min, Resuspend dry pelet in solubilizelysis buffer (with carbamide and detergent NP-40), Mix lysate with reducing SDS-PAGE (+merkaptoethanol) sample buffer, Reducing conditions. Positive control: HeLa human cell line. Immunohistochemistry on paraffin sections: 20 mg/mL, 0,1 % pepsin, 30 min, 37C, 4Covernight, chromogen DAB. Positive tissue: pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH ~7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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别名
- Cytokeratin 7
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背景
- Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin
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基因ID
- 3855
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UniProt
- P08729
抗原
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