Cytokeratin 18 抗体
(7 references)Quick Overview for Cytokeratin 18 抗体 (ABIN120626)
抗原
See all Cytokeratin 18 (KRT18) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody DA-7 reacts with Cytokeratin 18 (45 kDa).
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- Human breast carcinoma cell line PMC-42
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亚型
- IgG1
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anti-Keratin 18 (KRT18) (AA 53-150) antibody
KRT18 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 人 WB, ELISA, IHC (p), IP, ICC, ICFC 宿主: 小鼠 Monoclonal DC-10 unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 哺乳动物 WB, ELISA, IHC (p), IP, ICC, ICFC 宿主: 小鼠 Monoclonal C-04 unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 1-423) antibodyKRT18 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal E4 unconjugated
anti-Keratin 18 (KRT18) (AA 72-380) antibodyKRT18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 102-396) antibodyKRT18 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 人 WB, IHC, IF, FACS, StM, ISt 宿主: 兔 Monoclonal KRT18-2808R unconjugated Recombinant Antibody
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 人, 犬, Cat WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal DE-K18 unconjugated
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应用备注
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Immunoprecipitation. Western Blot: 1-2 mg/mL, overnight in 4 °C, Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail), Incubate 60 min on ice, Centrifuge to remove cell debris, Mix lysate with non-reducing SDS-PAGE sample buffer. Positive control: HeLa human cervix carcinoma cell line, A-431 human epidermoid carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry on paraffin sections: 1:10 - 1:100. Positive tissue: epithelial tissue (skin). Immunocytochemistry. Positive control: MCF-7 human breast adenocarcinoma cell line HeLa human cervix carcinoma cell line. ELISA: The antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04. Positive control: MCF-7 human breast adenocarcinoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH ~7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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: "Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, (2011) (PubMed).
: "Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells." in: Biochemical and biophysical research communications, Vol. 243, Issue 3, pp. 706-10, (1998) (PubMed).
: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).
: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).
: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).
: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).
: "Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte ..." in: Folia biologica, Vol. 32, Issue 1, pp. 12-25, (1986) (PubMed).
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: "Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, (2011) (PubMed).
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- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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别名
- Cytokeratin 18
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背景
- Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
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基因ID
- 3875
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UniProt
- P05783
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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