CD28 抗体 (Biotin)
Quick Overview for CD28 抗体 (Biotin) (ABIN120437)
抗原
See all CD28 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody SM268B recognizes a 90 kDa homeodimeric cell surface glycoprotein (CD28). Results of tissue Distribution by Flow Cytometry Analysis: Rat strain: Fischer Cell concentration: 1x10e6 cells per test Antibody concentration used: 1.0 μg/10e6 cells Isotypic control: Biotin Mouse IgG1 Cell source percentage of cells stained above control Thymus: 25.4 % Splenic T Cells*: 73.2 % *(T cells isolated with a Rat T Cell Recovery Column).
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纯化方法
- Protein G Chromatography
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免疫原
- Rat CD28 transfected A20J cells
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亚型
- IgG1
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) (AA 19-152) antibodyCD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow cytometry (for details please see Protocols below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This clone can costimulate T cell proliferation and IL-2 secretion by resting rat T cells. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
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有效期
- 6 months
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- CD28
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别名
- CD28
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背景
- CD28 has been found to be a potent costimulatory receptor on T cells. It is expressed on all peripheral rat alpha/beta and most gamma/delta T cells, as well as on approximately half of all NK cells.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
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基因ID
- 25660
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NCBI登录号
- NP_037253
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UniProt
- P31042
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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