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CD34 抗体 (FITC)

CD34 适用: 犬 FACS 宿主: 小鼠 Monoclonal 1H6 FITC
产品编号 ABIN120286
发货至: 中国
  • 抗原 See all CD34 抗体
    CD34
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    小鼠
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    This CD34 antibody is conjugated to FITC
    应用范围
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    Flow Cytometry (FACS)
    特异性
    SM2257F is specific for Canine CD34, a cell surface antigen of approximately 110 kDa expressed by endothelial cells and haematopoietic stem cells. Clone 1H6 is reported for use in CD34+ enrichment studies (4,5).
    纯化方法
    Affinity Chromatography on Protein G.
    免疫原
    Canine CD34 fusion protein. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1/FOX-NY myeloma cell line.
    克隆位点
    1H6
    亚型
    IgG1
    Top Product
    Discover our top product CD34 Primary Antibody
  • 应用备注
    Flow cytometry (neat - 1/10): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    CD34
    别名
    CD34 (CD34 产品)
    别名
    AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody
    背景
    The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
    基因ID
    415130
    NCBI登录号
    NP_001003341
    UniProt
    Q28270
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