Aurora A 抗体
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北京 101111
Quick Overview for Aurora A 抗体 (ABIN119942)
抗原
See all Aurora A (AURKA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises Aurora-A kinase. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.
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纯化方法
- Affinity chromatography on Protein G
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免疫原
- Recombinant Aurora-A. Spleen cells from immunised Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
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亚型
- IgG2b
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应用备注
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Immunoprecipitation. Western Blot: 1/500 - 1/1000, this antibody detects a band of approximately 46 kDa in human HeLa and mouse M-ICc12 cell lysates. Immunofluorescence. Recommended Positive Control: Human 293 and mouse LLC1 cell lines.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Aurora A (AURKA) (Aurora Kinase A (AURKA))
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别名
- Aurora Kinase A
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背景
- Aurora-A kinase is a 46 kDa serine/threonine kinase involved in mitotic spindle assembly. Aurora-A is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumourigenesis.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6
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基因ID
- 6790
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NCBI登录号
- NP_003591
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UniProt
- O14965
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途径
- Cell Division Cycle, Asymmetric Protein Localization
抗原
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