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Aurora A 抗体

The 小鼠 单克隆 anti-Aurora A antibody is suitable to detect Aurora A in samples from 人 和 小鼠. It has been validated for WB, IF 和 IP.
产品编号 ABIN119942
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Aurora A 抗体 (ABIN119942)

抗原

See all Aurora A (AURKA) 抗体
Aurora A (AURKA) (Aurora Kinase A (AURKA))

适用

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人, 小鼠

宿主

  • 124
  • 21
  • 1
  • 1
小鼠

克隆类型

  • 105
  • 41
单克隆

标记

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  • 1
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This Aurora A antibody is un-conjugated

应用范围

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  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

35C1
  • 特异性

    This antibody recognises Aurora-A kinase. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.

    纯化方法

    Affinity chromatography on Protein G

    免疫原

    Recombinant Aurora-A. Spleen cells from immunised Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.

    亚型

    IgG2b
  • 应用备注

    Immunoprecipitation. Western Blot: 1/500 - 1/1000, this antibody detects a band of approximately 46 kDa in human HeLa and mouse M-ICc12 cell lysates. Immunofluorescence. Recommended Positive Control: Human 293 and mouse LLC1 cell lines.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS, pH 7.4 containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Aurora A (AURKA) (Aurora Kinase A (AURKA))

    别名

    Aurora Kinase A

    背景

    Aurora-A kinase is a 46 kDa serine/threonine kinase involved in mitotic spindle assembly. Aurora-A is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumourigenesis.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6

    基因ID

    6790

    NCBI登录号

    NP_003591

    UniProt

    O14965

    途径

    Cell Division Cycle, Asymmetric Protein Localization
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