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c-MYC 抗体 (C-Term)

MYC 适用: 人 WB, FACS, IHC (p), IP, IHC (fro), EIA 宿主: 小鼠 Monoclonal 9-00E-010 unconjugated
产品编号 ABIN119684
发货至: 中国
  • 抗原 See all c-MYC (MYC) 抗体
    c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
    抗原表位
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    C-Term
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This c-MYC antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    序列
    EQKLISEEDL
    特异性
    This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-myc tag.
    交叉反应 (详细)
    Species reactivity (tested):Human.
    纯化方法
    Affinity Chromatography on Protein G.
    免疫原
    Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.
    克隆位点
    9-00E-010
    亚型
    IgG1
    Top Product
    Discover our top product MYC Primary Antibody
  • 应用备注
    ELISA: 1/100-1/500. Western Blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 1x10^6 cells in 100 μL. Membrane permeabilisation is required. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining. It does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS containing 0.09 % Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
    别名
    C-Myc (MYC 产品)
    背景
    P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag
    基因ID
    4609
    NCBI登录号
    NP_002458
    UniProt
    P01106
    途径
    p53 Pathway, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
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