c-MYC 抗体 (C-Term) (HRP)
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Quick Overview for c-MYC 抗体 (C-Term) (HRP) (ABIN119683)
抗原
See all c-MYC (MYC) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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序列
- AEEQKLISEE DLL
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特异性
- This antibody detects the 62 kDa c-myc gene product. SM1863HRP may also be used to detect the commonly used c-myc 'tag'.
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Synthetic peptide sequence corresponding to the C-terminal region of Human c-myc.
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亚型
- IgG1
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应用备注
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ELISA: 1/100-1/500. Western blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining and does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 0.01 % Thimerosal, HRP Stabilizer
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
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别名
- C-Myc
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背景
- C-myc gene product is involved in the regulation of the cell cycle and cell growth. It is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag
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基因ID
- 4609
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NCBI登录号
- NP_002458
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UniProt
- P01106
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途径
- p53 Pathway, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
抗原
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