电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

MCAM 抗体 (N-Term) (FITC)

This anti-MCAM antibody is a 小鼠 单克隆 antibody detecting MCAM in FACS, IHC (fro) 和 EIA. Suitable for 人 和 Pig. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN119672
发货至: 中国

Quick Overview for MCAM 抗体 (N-Term) (FITC) (ABIN119672)

抗原

See all MCAM 抗体
MCAM (Melanoma Cell Adhesion Molecule (MCAM))

适用

  • 174
  • 55
  • 54
  • 15
  • 15
  • 8
  • 7
  • 6
  • 5
  • 2
人, Pig

宿主

  • 108
  • 101
  • 2
  • 1
  • 1
  • 1
小鼠

克隆类型

  • 115
  • 99
单克隆

标记

  • 98
  • 17
  • 13
  • 9
  • 8
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MCAM antibody is conjugated to FITC

应用范围

  • 111
  • 98
  • 78
  • 42
  • 33
  • 30
  • 28
  • 24
  • 12
  • 11
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

克隆位点

OJ79c
  • 抗原表位

    • 24
    • 16
    • 9
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-5, N-Term

    特异性

    This antibody recognises the 118 kDa cell surface glycoprotein CD146, also known as MUC18, Mel-CAM and S-endo. CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells.

    纯化方法

    Affinity Chromatography on Protein G

    免疫原

    Recombinant Human MUC18 (D1-D5) Fc protein. Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.

    亚型

    IgG1
  • 应用备注

    Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Clone OJ79c (purified antibody) has been successfully used in ELISA and immunohistochemistry on frozen sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.09 % Sodium Azide, 1 % BSA

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Möbius-Winkler, Hilberg, Menzel, Golla, Burman, Schuler, Adams: "Time-dependent mobilization of circulating progenitor cells during strenuous exercise in healthy individuals." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 107, Issue 6, pp. 1943-50, (2010) (PubMed).

  • 抗原

    MCAM (Melanoma Cell Adhesion Molecule (MCAM))

    别名

    CD146

    背景

    CD146 is a member of the immunoglobulin superfamily, expressed by all endothelial cells and by melanoma cells. CD146 appears to act as an adhesion molecule. Expression in melanoma may be linked to disease progression.Synonyms: A32, CD146, Cell surface glycoprotein P1H12, MCAM, MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

    基因ID

    4162

    NCBI登录号

    NP_006491

    UniProt

    P43121
You are here:
Chat with us!