Myeloperoxidase 抗体
(2 references)Our Local Distributor
北京 101111
Quick Overview for Myeloperoxidase 抗体 (ABIN119048)
抗原
See all Myeloperoxidase (MPO) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
纯化方法
- Affinity Chromatography on Protein G
-
免疫原
- Human Myeloperoxidase. Spleen cells from immunised mice were fused with cells of the mouse X63 AG8653 myeloma cell line.
-
亚型
- IgG1
-
-
anti-Myeloperoxidase (MPO) (AA 406-745) antibody
MPO 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 134-263) antibodyMPO 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 50-310) antibodyMPO 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 646-745) antibodyMPO 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E11 unconjugated
anti-Myeloperoxidase (MPO) (AA 406-745) antibodyMPO 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 678-745) antibodyMPO 适用: 人, 小鼠, 大鼠 ELISA, ICC, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 253-718) antibodyMPO 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) antibodyMPO 适用: 人 IHC 宿主: 小鼠 Monoclonal 692M unconjugated
anti-Myeloperoxidase (MPO) (AA 714-728), (C-Term) antibodyMPO 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 165-278) antibodyMPO 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
-
-
应用备注
-
Flow Cytometry (1/50-1/100): Membrane permeabilisation is required. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry. Immunhistology on Frozen Sections (1/1000-1/5000). Immunhistology on Paraffin Sections (1/500-1/1000): use bone marrow as positive control. ELISA. Western blotting (Non reducing conditions): ABIN119048 recognises native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognises recombinant MPO in Western blots and weakly in ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- PBS containing 0.09 % Sodium Azide as preservative
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
-
: "Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies." in: Scientific reports, Vol. 5, pp. 11888, (2015) (PubMed).
: "C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 20, Issue 2, pp. 289-98, (2009) (PubMed).
-
: "Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies." in: Scientific reports, Vol. 5, pp. 11888, (2015) (PubMed).
-
- Myeloperoxidase (MPO)
-
别名
- Myeloperoxidase
-
背景
- Myeloperoxidase/MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kD) and two light chains (15 kD), the heavy chains being linked by a disulphide bond. Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59-64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase auto-antibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.Synonyms: MPO
-
基因ID
- 9606
-
UniProt
- P05164
-
途径
- Chromatin Binding
抗原
-
