Cyclin D1 抗体
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- 抗原 See all Cyclin D1 (CCND1) 抗体
- Cyclin D1 (CCND1)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cyclin D1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody recognises the Cyclin D1.
- 纯化方法
- Affinity Chromatography on Protein A.
- 免疫原
- Human Cyclin D1. Spleen cells from immunised mice were fused with cells of the mouse Sp-2 myeloma cell line.
- 克隆位点
- CD1-1
- 亚型
- IgG1
- Top Product
- Discover our top product CCND1 Primary Antibody
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anti-Cyclin D1 (CCND1) (AA 61-110) antibody
CCND1 适用: 人, 小鼠, 大鼠, Cow, 兔, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) antibodyKO Validated CCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Cyclin D1 (CCND1) (AA 1-295) antibodyCCND1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1F7F5 unconjugated
anti-Cyclin D1 (CCND1) (AA 167-295) antibodyCCND1 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 3B6G3 unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (AA 68-97) antibodyCCND1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB8066 unconjugated
anti-Cyclin D1 (CCND1) (AA 68-97) antibodyCCND1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) antibodyCCND1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal DCS-6 unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow Cytometry: Membrane permeabilisation is required for this application. ELISA. Immunoprecipitation. Western Blot: 1/500. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/1000 - 1/10000, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Small intestine, colon.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Cyclin D1 (CCND1)
- 别名
- Cyclin D1 (CCND1 产品)
- 背景
- Cyclin D1 a 36kD nuclear protein. D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy, and over expression has been linked to poor prognosis. During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. In a recent study, Cyclin D1 regulates cellular metabolism, fat cell differentiation and cellular migration. Cyclin D1 is also involved in development and cancer.Synonyms: BCL-1 oncogene, BCL1, CCND1, Cyclin-D1, G1/S-specific cyclin-D1, PRAD-1 oncogene, PRAD1
- 基因ID
- 595
- NCBI登录号
- NP_444284
- UniProt
- P24385
- 途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
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