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EGFR 抗体 (Extracellular Domain)

Name: EGFR 抗体 (Extracellular Domain)
SKU: ABIN118950
Availability: OutOfStock

EGFR 适用: 人 WB, FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal ICR10 unconjugated

Staining of A431 cells with RAT ANTI HUMAN EGF RECEPTOR (ABIN118720) visualised with F(ab')2 RABBIT ANTI RAT IgG:FITC (EGFR 抗体 (Extracellular Domain))

1 image

产品编号 ABIN118950

发货至: 中国

Datasheet as PDF

抗原 See all EGFR 抗体

EGFR (Epidermal Growth Factor Receptor (EGFR))
抗原表位
39

38

31

31

29

27

27

26

24

22

19

18

16

16

16

15

15

15

11

11

10

9

9

9

8

8

7

6

5

5

5

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3
Extracellular Domain
适用
744

296

283

25

25

24

19

14

8

3

2

2

1

1

1

1

1

1
人
宿主
579

167

13

13

5

1

1
大鼠
Compatible Secondaries
克隆类型
541

233

2
单克隆
标记
406

58

29

22

19

17

16

16

16

16

16

11

10

10

10

10

10

10

10

10

10

8

8

5

4

4

4

4

4

1

1

1

1

1

1
This EGFR antibody is un-conjugated
应用范围
532

243

147

142

127

102

99

93

82

46

37

34

33

18

11

8

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

This antibody recognises the epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. This antibody binds to epitope B from EGFR (1, 2) and has an affinity of 6.7 x 10e-9 M.

纯化方法

Ion exchange chromatography

免疫原

Extracellular domain of human Epidermal Growth Factor Receptor (EGFR) from head and neck carcinoma.

克隆位点

ICR10

亚型

IgG2a

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anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibody
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 960-1060) antibody
EGFR 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibody
EGFR 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibody
EGFR 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5E10D3 unconjugated

应用备注

Immunoprecipitation. Western blotting. Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Recommended positive control: Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, pH 7.4 containing 0.09 % Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原

EGFR (Epidermal Growth Factor Receptor (EGFR))

别名

EGFR / ERBB1 (EGFR 产品)

背景

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

基因ID

1956

NCBI登录号

NP_005219

UniProt

P00533

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins