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C5AR1 抗体 (N-Term)

This anti-C5AR1 antibody is a 小鼠 单克隆 antibody detecting C5AR1 in WB, FACS 和 IHC (p). Suitable for 人, Cow 和 兔.
产品编号 ABIN118843
发货至: 中国

Quick Overview for C5AR1 抗体 (N-Term) (ABIN118843)

抗原

See all C5AR1 抗体
C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

适用

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人, Cow, 兔

宿主

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小鼠

克隆类型

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单克隆

标记

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This C5AR1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

S5-1
  • 抗原表位

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    AA 1-31, N-Term

    特异性

    This antibody recognises the C5a receptor (C5aR) CD88. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.

    交叉反应 (详细)

    Species reactivity (expected):Bovine and Rabbit.
    Species reactivity (tested):Human

    纯化方法

    Ammonium sulphate precipitation

    免疫原

    C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.

    亚型

    IgG2a
  • 应用备注

    Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL. Immunohistochemistry on paraffin sections. Western Blot. Functional Assays.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS containing 0.09 % Sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

    别名

    CD88 / C5R1

    背景

    CD88 is predominantly expressed on cells of the myeloid lineage.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1

    基因ID

    728

    NCBI登录号

    NP_001727

    UniProt

    P21730

    途径

    Complement System
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