C5AR1 抗体 (N-Term)
Our Local Distributor
北京 101111
Quick Overview for C5AR1 抗体 (N-Term) (ABIN118843)
抗原
See all C5AR1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 1-31, N-Term
-
特异性
- This antibody recognises the C5a receptor (C5aR) CD88. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.
-
交叉反应 (详细)
-
Species reactivity (expected):Bovine and Rabbit.
Species reactivity (tested):Human -
纯化方法
- Ammonium sulphate precipitation
-
免疫原
- C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
-
亚型
- IgG2a
-
-
-
-
应用备注
-
Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL. Immunohistochemistry on paraffin sections. Western Blot. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- PBS containing 0.09 % Sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
-
别名
- CD88 / C5R1
-
背景
- CD88 is predominantly expressed on cells of the myeloid lineage.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1
-
基因ID
- 728
-
NCBI登录号
- NP_001727
-
UniProt
- P21730
-
途径
- Complement System
抗原
-