(1 reference)-
- 抗原 See all CD55 抗体
- CD55 (Complement Decay-Accelerating Factor (CD55))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD55 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography on Protein G.
- 免疫原
- K562 cells
- 克隆位点
- 67
- 亚型
- IgG1
- Top Product
- Discover our top product CD55 Primary Antibody
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anti-Complement Decay-Accelerating Factor (CD55) (AA 35-134) antibody
CD55 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1G3 unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 35-285) antibodyCD55 适用: 人 WB, ELISA, IHC (fro), ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 40-300) antibodyCD55 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 51-79) antibodyCD55 适用: 人 WB, FACS, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 35-134) antibodyCD55 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (Center) antibodyCD55 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 254-372) antibodyCD55 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) (AA 35-126) antibodyCD55 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Decay-Accelerating Factor (CD55) antibodyCD55 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 1A5 unconjugated Recombinant Antibody
anti-Complement Decay-Accelerating Factor (CD55) antibodyCD55 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal F4-29D9 unconjugated
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- 应用备注
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Flow Cytometry: Use 10 μL of 1/10-1/25 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control Tissue: Human Tonsil. (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100using heat treatment for antigen retrieval) was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4, with 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, (2005) (PubMed).
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: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, (2005) (PubMed).
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- 抗原
- CD55 (Complement Decay-Accelerating Factor (CD55))
- 别名
- CD55 / DAF (CD55 产品)
- 别名
- CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody
- 背景
- CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin. CD55 is expressed by a wide range of cell types.Synonyms: Complement decay-accelerating factor
- 基因ID
- 9606
- UniProt
- P08174
- 途径
- Complement System, Regulation of Leukocyte Mediated Immunity
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