FUT4
适用: 人
FACS, IP
宿主: 小鼠
Monoclonal
MEM-158
Biotin
应用备注
In flow cytometry (1.0 x 10^6 cells ), IFA and IHC (acetone-fixed frozen sections). May be used for enumerating mature granulocytes in peripheral blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
100 µg/mL (OD280nm)
缓冲液
0.01 M PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
The CD15 antigen is expressed on approximately 90 % human circulating granulocytes (membranes and granules), 30 to 60 % of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.Synonyms: Lacto-N-Fucopentaose III, Lewis X, SSEA1, Stage-Specific Embryonic Antigen, X-Hapten