LIM Domain Binding 1 Protein 抗体 (AA 361-373)
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Quick Overview for LIM Domain Binding 1 Protein 抗体 (AA 361-373) (ABIN118013)
抗原
See all LIM Domain Binding 1 Protein (LDB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 361-373
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特异性
- This affinity purified antibody is directed against the mouse LDB1protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, frog, chicken and rat based on 100 % homology for the immunogen sequence. Cross reactivity with LDB1 homologues from other sources has not been determined.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 361-373 of mouse LDB1 protein.
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anti-LIM Domain Binding 1 (LDB1) antibody
KO Validated LDB1 适用: 人 WB, IP, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 1-50) antibodyLDB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) antibodyLDB1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 1-110) antibodyLDB1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 303-352) antibodyLDB1 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 豚鼠, 马, 兔, 小鸡, 非洲爪蟾, Pig, Bat, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 50-375) antibodyLDB1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 336-368), (C-Term) antibodyLDB1 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB47030 unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 1-110) antibodyLDB1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 251-300) antibodyLDB1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 小鸡, 非洲爪蟾, Pig, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a bandapproximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriatecell lysate or extract. This antibody has been used in a ChIP assay using murineerythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1gene, a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was usedsuccessfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20 g for 10e8 cells for ChIP. ELISA: 1: 2,000-1: 8,000. Western Blot: 1: 500-1: 3,000. Immunofluorescence microscopy.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 2-8 °C.
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- LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
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别名
- LDB1
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背景
- LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.Synonyms: CLIM-2, CLIM2, Carboxyl-terminal LIM domain-binding protein 2, LDB1, LIM domain-binding factor CLIM2, LIM domain-binding protein 1, Nuclear LIM interactor, hLdb1
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基因ID
- 16825
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NCBI登录号
- NP_001106879
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UniProt
- P70662
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途径
- Stem Cell Maintenance, Chromatin Binding
抗原
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