PLK1 抗体 (pThr210)
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Quick Overview for PLK1 抗体 (pThr210) (ABIN117982)
抗原
See all PLK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 205-214, pThr210
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序列
- 1 msaavtagkl arapadpgka gvpgvaapga paaappakei pevlvdprsr rryvrgrflg 61 kggfakcfei sdadtkevfa gkivpkslll kphqrekmsm eisihrslah qhvvgfhgff 121 edndfvfvvl elcrrrslle lhkrrkalte pearyylrqi vlgcqylhrn rvihrdlklg 181 nlflnedlev kigdfglatk veydgerkkt lcgtpnyiap evlskkghsf evdvwsigci 241 mytllvgkpp fetsclkety lrikkneysi pkhinpvaas liqkmlqtdp tarptinell 301 ndefftsgyi parlpitclt ipprfsiaps sldpsnrkpl tvlnkglenp lperprekee 361 pvvretgevv dchlsdmlqq lhsvnaskps erglvrqeea edpacipifw vskwvdysdk 421 yglgyqlcdn svgvlfndst rlilyndgds lqyierdgte syltvsshpn slmkkitllk 481 yfrnymsehl lkaganitpr egdelarlpy lrtwfrtrsa iilhlsngsv qinffqdhtk 541 lilcplmaav tyidekrdfr tyrlslleey gcckelasrl ryartmvdkl lssrsasnrl 601 kas
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特异性
- This antibody is specific for phosphorylated Human Plk-1 protein at the pThr210 residue. Cross reactivity with Plk-1 protein homologues from C.elgans and Honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
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交叉反应 （详细）
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Species reactivity (expected):Chimpanzee, Pig, Chicken, Mouse, Rat, Xenopus, Dog, Mosquito, Zebrafish, Starfish, Sea urchin and Fruit Fly.
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity Chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities
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免疫原
- Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein
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anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibody
PLK1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 304-603) antibodyPLK1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibodyPLK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4G11 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibodyPLK1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 5C1 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人, 犬 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6C6 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人, 猴, 犬 WB, IF, IP, FACS 宿主: 小鼠 Monoclonal 8C12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 201-300) antibodyPLK1 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 3F12 unconjugated
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应用备注
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ELISA (1/3,000-1/12,000). Immunohistochemistry on Paraffin Sections (1/200-1/1,000). This antibody is positive byIHC on kidney, liver, cancer, thyroid and lymphocyte tissue. Western blot (1/200-1/2,000). Expect a band approximately 68 kDa in size correspondingto Plk-1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PLK1 (Polo-Like Kinase 1 (PLK1))
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别名
- PLK1
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背景
- Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.Synonyms: PLK-1, Polo-like kinase 1, STPK13, Serine/threonine-protein kinase PLK1
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基因ID
- 5347
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NCBI登录号
- NP_005021
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UniProt
- P53350
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途径
- Cell Division Cycle, M Phase
抗原
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