Myeloperoxidase 抗体
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北京 101111
Quick Overview for Myeloperoxidase 抗体 (ABIN117976)
抗原
See all Myeloperoxidase (MPO) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below.
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免疫原
- Myeloperoxidase [Human Leukocytes]
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anti-Myeloperoxidase (MPO) (AA 406-745) antibody
MPO 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 134-263) antibodyMPO 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 646-745) antibodyMPO 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E11 unconjugated
anti-Myeloperoxidase (MPO) (AA 50-310) antibodyMPO 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 406-745) antibodyMPO 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 678-745) antibodyMPO 适用: 人, 小鼠, 大鼠 ELISA, FACS, ICC, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 253-718) antibodyMPO 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) antibodyMPO 适用: 人 IHC 宿主: 小鼠 Monoclonal 692M unconjugated
anti-Myeloperoxidase (MPO) (AA 714-728), (C-Term) antibodyMPO 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Myeloperoxidase (MPO) (AA 165-278) antibodyMPO 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
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应用备注
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This antibody is suitable for western blotting, ELISA (1: 200,000) and Immunoprecipitation. Although not tested, this antibody is likely functional in Immunohistochemistry and otherimmunological methods. This product was assayed by Immunoblot and was found to bereactive with human myeloperoxidase isolated from leukocytes. The blot was probed withImmunochemical's anti-Myeloperoxidase [Human Leukocytes] used at a dilution of 1: 2000. Use Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT) as a secondaryantibody for detection. The antibody detects a multiple bands corresponding to 53 kDa and15 kDa polypeptides and possibly other chain combination (68 kDa and 106 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
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浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Gentamicin Sulfate as preservative.
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at RT. This product is stable for one month at 2-8 °C as an undiluted liquid.
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- Myeloperoxidase (MPO)
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别名
- Myeloperoxidase
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背景
- Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5 % . MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.Synonyms: MPO
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基因ID
- 9606
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UniProt
- P05164
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途径
- Chromatin Binding
抗原
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