POLM 抗体 (AA 6-18)
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Quick Overview for POLM 抗体 (AA 6-18) (ABIN117919)
抗原
See all POLM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 6-18
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序列
- Peptide sequence: R-R-A-R-V-G-S-P-S-G-D-A-A-C
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特异性
- This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 6-18 of Human POLM µ (DNA polymerase mu). POLM µ is a template-directed terminal deoxynucleotidetransferase that shows a preferential expression in proliferating lymphoid cells.
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应用备注
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This affinity purified antibody has been tested for use in ELISA against the immunizingpeptide. Reactivity in other immunoassays is unknown. This product has been assayed byELISA against 0.1 μg of the immunizing peptide. A 1: 33,000 to 1: 135,000 dilution of the antibody is recommended for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 2.1 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
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- POLM (Polymerase (DNA Directed), mu (POLM))
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别名
- DNA Polymerase mu
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背景
- DNA ploymerase mu is a template directed terminal deoxynucleotidetransferase that shows a preferential expression in proliferating lymphoid cells. It seems to act as an Ig mutase which is responsible for immunoglobulin (Ig) gene hypermutation.Synonyms: POLM, Pol Mu, Tdt-N, TdtN
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基因ID
- 27434
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NCBI登录号
- NP_037416
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UniProt
- Q9NP87
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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