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Phosphotyrosine 抗体

Cited in 3+ publications. The 小鼠 单克隆 anti-Phosphotyrosine antibody (Clone PY20) (ABIN1177365) specifically detects Phosphotyrosine in ICS.
产品编号 ABIN1177365
发货至: 中国
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Quick Overview for Phosphotyrosine 抗体 (ABIN1177365)

抗原

Phosphotyrosine

宿主

  • 100
  • 42
  • 1
小鼠

克隆类型

  • 133
  • 10
单克隆

标记

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This Phosphotyrosine antibody is un-conjugated

应用范围

  • 88
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Intracellular Staining (ICS)

克隆位点

PY20
  • 品牌

    BD Phosflow™

    纯化方法

    Purified from tissue culture supernatant or ascites by affinity chromatography

    亚型

    IgG2b
  • 样本量

    20 μL

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
  • Arvidsson, Rupp, Nånberg, Downward, Rönnstrand, Wennström, Schlessinger, Heldin, Claesson-Welsh: "Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation." in: Molecular and cellular biology, Vol. 14, Issue 10, pp. 6715-26, (1994) (PubMed).

    Fan, Mendelsohn, Masui, Kumar: "Regulation of epidermal growth factor receptor in NIH3T3/HER14 cells by antireceptor monoclonal antibodies." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21073-9, (1993) (PubMed).

    Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, (1988) (PubMed).

  • 抗原

    Phosphotyrosine

    物质类

    Amino Acid

    背景

    Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.
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