IL4 Receptor 抗体
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北京 101111
Quick Overview for IL4 Receptor 抗体 (ABIN1177312)
抗原
See all IL4 Receptor (IL4R) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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Neutralization Activity: This antibody has been reported to be useful for neutralizing the action of recombinant mouse IL-4. Neutralization activity may be measured with a proliferation assay using 200 pg/mL recombinant mouse IL-4 (Cat. No. 550067) to stimulate D36 cells at 1x10^5 cells/mL as indicator cells.
50% Neutralization (ND50) at 2-20 ng/mL
>95% Neutralization at 1-10 myg/mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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亚型
- IgG2a kappa
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anti-Interleukin 4 Receptor (IL4R) (AA 26-232) antibody
IL4R 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 Receptor (IL4R) (AA 26-233) antibodyIL4R 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 Receptor (IL4R) (AA 201-300) antibodyIL4R 适用: 人, 小鼠 WB, ELISA, FACS, ICC, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 Receptor (IL4R) (AA 26-232) antibodyIL4R 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4B12B12 unconjugated
anti-Interleukin 4 Receptor (IL4R) (N-Term) antibodyIL4R 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 Receptor (IL4R) (AA 1-227) antibodyIL4R 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 4 Receptor (IL4R) antibodyIL4R 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 Receptor (IL4R) (Ser540) antibodyIL4R 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 Receptor (IL4R) antibodyIL4R 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 Receptor (IL4R) (AA 677-691), (C-Term) antibodyIL4R 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB48074 unconjugated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Biologic functions and signaling of the interleukin-4 receptor complexes." in: Immunobiology, Vol. 201, Issue 3-4, pp. 285-307, (2000) (PubMed).
: "CD28 costimulation accelerates IL-4 receptor sensitivity and IL-4-mediated Th2 differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2432-42, (1999) (PubMed).
: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, (1997) (PubMed).
: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, (1994) (PubMed).
: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, (1991) (PubMed).
: "Monoclonal antibodies block murine IL-4 receptor function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4212-7, (1990) (PubMed).
: "The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms." in: Cell, Vol. 59, Issue 2, pp. 335-48, (1989) (PubMed).
: "Expression of high affinity receptors for murine interleukin 4 (BSF-1) on hemopoietic and nonhemopoietic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 2, pp. 456-64, (1988) (PubMed).
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: "Biologic functions and signaling of the interleukin-4 receptor complexes." in: Immunobiology, Vol. 201, Issue 3-4, pp. 285-307, (2000) (PubMed).
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- IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))
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别名
- CD124
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背景
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The mIL4R-M1 monoclonal antibody specifically binds to CD124 which is also known as the alpha subunit of the mouse Interleukin-4 Receptor (IL-4Ralpha). The mouse IL-4Ralpha is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Ralpha chain binds IL-4 with high affinity and associates with either the common gamma chain (IL-4Ralpha/gammac, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Ralpha/IL-13Ralpha, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4’s biological activities. The mIL4R-M1 antibody also recognizes naturally-occurring, soluble truncated forms of IL-4Ralpha (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Ralpha domain or from differential mRNAsplicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R.
Synonyms: IL-4 Receptor alpha chain -
途径
- JAK/STAT Signaling, Positive Regulation of Immune Effector Process
抗原
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