IL-10 抗体
(2 references)Quick Overview for IL-10 抗体 (ABIN1177290)
抗原
See all IL-10 (IL10) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay using 2.0 ng/mL recombinant mouse IL-10 and 50 pg/mL recombinant mouse IL-4 to costimulate D36 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-10 for 60 minutes).
50% Neutralization (ND50) at 2.5 - 20 ng/mL
>95% Neutralization at 30 - 125 ng/mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Recombinant mouse IL-10
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亚型
- IgG2b
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anti-Interleukin 10 (IL10) (AA 101-178) antibody
IL10 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人, 小鼠, 大鼠, Cow, 兔, Pig, 马, 犬, 猴, 小鸡, 山羊, 绵羊, 豚鼠, Avian, Cat, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB971550 unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB312926 unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 10 (IL10) (AA 27-53) antibodyIL10 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
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应用备注
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ELISA: Biotinylated JES5-16E3 antibody (Cat. No. 554465) is useful as a detection antibody for sandwich ELISA for measuring mouse IL-10 protein levels and can be paired with purified JES5-2A5 antibody as the capture antibody along with recombinant mouse IL-10 (Cat. No. 550070) as the standard. For testing mouse IL-10 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the Mouse IL-10 BD OptEIA™ ELISA Set (Cat. No. 555252).
Flow Cytometry: The JES5-16E3 antibody is useful for flow cytometric analysis to identify and enumerate IL-10 producing cells within mixed cell populations. Direct conjugates of the JES5-16E3 antibody are especially suitable for these experiments, such as PE Rat Anti-Mouse IL-10 (Cat. No. 554467). -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, (1994) (PubMed).
: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).
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: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, (1994) (PubMed).
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- IL-10
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背景
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The JES5-16E3 monoclonal antibody specifically binds to the mouse cytokine, Interleukin-10 (IL-10). IL-10 is also known as Cytokine Synthesis Inhibitory Factor (CSIF). It is produced by various activated cell types including CD4+ T cells, CD8+ T cells, NK T cells, B1 B cells, NK cells, macrophages, dendritic cells, mast cells, granulocytes and keratinocytes. IL-10 plays a pivotal role in regulating immune responses and protecting the host from damage caused by inflammatory and autoimmune responses. IL-10 has numerous biological activities including the inhibition of cytokine synthesis by activated T cells, NK cells, monocytes, and macrophages. In the presence of accessory cells, IL-10 inhibits mitogen- or anti-CD3 induced proliferation of T lymphocytes. IL-10 has also been shown to costimulate the development of thymocytes, B cell differentiation and the generation of cytotoxic T cells. The immunogen used to generate the JES5-16E3 hybridoma was recombinant mouse IL-10. JES5-16E3 is a neutralizing antibody.
Synonyms: Interleukin-10, Il10, CSIF, Cytokine synthesis inhibitory factor -
途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原
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