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- 抗原 See all IL-4 (IL4) 抗体
- IL-4 (IL4) (Interleukin 4 (IL4))
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适用
- 人
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-4 antibody is un-conjugated
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应用范围
- ELISA, Neutralization (Neut), Intracellular Staining (ICS)
- 品牌
- BD Pharmingen™
- 产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-4. Neutralization may be measured with a proliferation assay using 2 ng/mL recombinant human IL-4 (Cat. No. 554605) to stimulate TF-1 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-4 for 60 minutes).
50% Neutralization (ND50) at 4 - 20 ng/mL
>95% Neutralization at 20 - 100 ng/mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Purified Recombinant Human IL-4
- 克隆位点
- MP4-25D2
- 亚型
- IgG1
- Top Product
- Discover our top product IL4 Primary Antibody
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- 应用备注
- ELISA: Biotinylated MP4-25D2 antibody (Cat. No. 554483) has been reported to be useful as a detection antibody for sandwich ELISA measuring human IL-4 protein levels. Biotinylated MP4-25D2 antibody can be paired with the purified 8D4-8 antibody (Cat. No. 554515) as the capture antibody, with recombinant human IL-4 (Cat. No. 554605) as the standard. Investigators are encouraged to use the BD OptEIA™ Human IL-4 ELISA (Cat. No. 555194 or 550614) for measuring human IL-4 in serum or plasma samples.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, (1993) (PubMed).
: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, (1989) (PubMed).
: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).
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Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
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- 抗原
- IL-4 (IL4) (Interleukin 4 (IL4))
- 别名
- IL-4 (IL4 产品)
- 别名
- IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody
- 背景
- The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described.
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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