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STAT5A 抗体 (pTyr694) (PE-CF594)

Name: STAT5A 抗体 (pTyr694) (PE-CF594)
SKU: ABIN1177255
Availability: OutOfStock

This anti-STAT5A antibody is a 小鼠单克隆 antibody detecting STAT5A in ICS. Suitable for 人. This Primary Antibody has been cited in 4+ publications.

(4 references)

产品编号 ABIN1177255

发货至: 中国

Quick Overview for STAT5A 抗体 (pTyr694) (PE-CF594) (ABIN1177255)

抗原

See all STAT5A 抗体

STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This STAT5A antibody is conjugated to PE-CF594

应用范围

Intracellular Staining (ICS)

克隆位点

47-Stat5

Discover our top product STAT5A Primary Antibody

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pTyr694

品牌

BD Phosflow™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Phosphorylated Sheep Stat5 Peptide

亚型

IgG1 kappa
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) antibody
STAT5A 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 6E5 unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 583-794) antibody
STAT5A 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6D4 unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 1-794) antibody
STAT5A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 1-104) antibody
STAT5A 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1B12 unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 635-794) antibody
STAT5A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (C-Term) antibody
STAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody
STAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pSer780) antibody
STAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 61-160) antibody
STAT5A 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 763-797), (C-Term) antibody
STAT5A 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB52392 unconjugated

应用备注

This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I).

说明

Signal transducer, activator of transcription 5, MGF, MPF

样本量

5 μL

限制

仅限研究用
状态

Liquid

缓冲液

Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.
Van De Wiele, Marino, Murray, Vo, Whetsell, Teague: "Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4235-44, (2004) (PubMed).

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).

Liu, Gaffen, Goldsmith: "JAK/STAT signaling by cytokine receptors." in: Current opinion in immunology, Vol. 10, Issue 3, pp. 271-8, (1998) (PubMed).

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, (1994) (PubMed).
抗原

STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

别名

Stat5

背景

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699. This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
Synonyms: Signal transducer and activator of transcription 5, MGF, MPF

途径

JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation