(3 references)-
- 抗原 See all CrkL (CRKL) 抗体
- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
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抗原表位
- pTyr207
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CrkL antibody is conjugated to PE
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应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Human, Mouse, and Rat CrkL Peptide
- 克隆位点
- K30-391-50-80
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CRKL Primary Antibody
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anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) antibody
CRKL 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 204-303) antibodyCRKL 适用: 人 WB, ELISA, IF, PLA, RNAi 宿主: 小鼠 Monoclonal 4B5 unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) antibodyCRKL 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5E12 unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (pTyr251) antibodyCRKL 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) antibodyCRKL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 201-303) antibodyCRKL 适用: 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 98-303) antibodyCRKL 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) antibodyCRKL 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (C-Term) antibodyVerified CRKL 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) antibodyCRKL 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This PE-conjugated antibody is suitable for intracellular staining of cell lines using BD Cytofix™ Fixation Buffer and BD Phosflow Perm Buffer III or IV. We have confirmed that the Alexa Fluor® 647 conjugate, Cat. No. 560790, is suitable for staining leukocytes from human peripheral blood and bone marrow.
- 说明
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BALB/c
- 样本量
- 20 μL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Crk family adaptors-signalling complex formation and biological roles." in: Oncogene, Vol. 20, Issue 44, pp. 6348-71, (2001) (PubMed).
: "CrkL activates integrin-mediated hematopoietic cell adhesion through the guanine nucleotide exchange factor C3G." in: Blood, Vol. 93, Issue 11, pp. 3713-22, (1999) (PubMed).
: "Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells." in: Molecular and cellular biology, Vol. 18, Issue 9, pp. 5082-90, (1998) (PubMed).
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: "Crk family adaptors-signalling complex formation and biological roles." in: Oncogene, Vol. 20, Issue 44, pp. 6348-71, (2001) (PubMed).
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- 抗原
- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
- 别名
- CrkL (CRKL 产品)
- 背景
- Crk-Like (CrkL) is an adaptor protein that is preferentially expressed in hematopoietic cells and is encoded by a gene that is homologous to the viral oncogene v-crk (chicken tumor virus no. 10 regulator of kinase). Its SH2 and SH3 domains bind to a variety of effector proteins, such as paxillin, p130Cas, c-Cbl, c-Abl, and C3G. These interactions are involved in the regulation of cellular migration, adhesion, and transformation. Tyrosine 207 (Y207) of CrkL is phosphorylated in hematopoietic cells that express the BCR-ABL fusion protein. This site may be a negative regulator of protein complex formation and biological activity. The K30-391.50.80 monoclonal antibody recognizes the phosphorylated Y207 of human CrkL.
- 途径
- Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor
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