Neutralization Activity: This antibody has been reported to be useful for the neutralization of mouse IFN-gamma anti-viral activity. Neutralization activity may be measured with a encphalomyocarditis viral (EMCV) resistance assay using 1 ng/mL recombinant mouse IFN-gamma (Cat. No. 554587) with L929 indicator cells. 50% Neutralization (ND50) at 1 - 20 ng/mL 95% Neutralization at 15 - 333 ng/mL
纯化方法
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
过滤
0.2 μm filtered
内毒素水平
Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.
储存液
Azide free
储存条件
4 °C
储存方法
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
Hsieh, Schrenzel, Mulvania, Lepper, DiMolfetto-Landon, Ferrick: "In vivo cytokine production in murine listeriosis. Evidence for immunoregulation by gamma delta+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 1, pp. 232-7, (1996) (PubMed).
Ferrick, Schrenzel, Mulvania, Hsieh, Ferlin, Lepper: "Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo." in: Nature, Vol. 373, Issue 6511, pp. 255-7, (1995) (PubMed).
Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, (1995) (PubMed).
Vikingsson, Pederson, Muller: "Enumeration of IFN-gamma producing lymphocytes by flow cytometry and correlation with quantitative measurement of IFN-gamma." in: Journal of immunological methods, Vol. 173, Issue 2, pp. 219-28, (1994) (PubMed).
Sarawar, Sangster, Coffman, Doherty: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, (1994) (PubMed).
Yang, HayGlass: "A simple, sensitive, dual mAb based ELISA for murine gamma interferon determination: comparison with two common bioassays." in: Journal of immunoassay, Vol. 14, Issue 3, pp. 129-48, (1993) (PubMed).
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
Green, Yeh, Overall: "Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).
The XMG1.2 monoclonal antibody specifically binds to mouse interferon-gamma (IFN-gamma) protein. IFN-gamma is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-gamma production is associated with the Th1 cell differentiation. The purified form of this antibody has been reported to be a neutralizing antibody. Synonyms: IFN-gamma, IFN-g, IFN-gamma, Interferon gamma, Type II Interferon