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CBL 抗体 (pTyr700) (PE)

Name: CBL 抗体 (pTyr700) (PE)
SKU: ABIN1176884
Availability: OutOfStock

This anti-CBL antibody is a 小鼠单克隆 antibody detecting CBL in ICS. Suitable for 人和小鼠. This Primary Antibody has been cited in 3+ publications.

(3 references)

产品编号 ABIN1176884

发货至: 中国

Datasheet as PDF

Quick Overview for CBL 抗体 (pTyr700) (PE) (ABIN1176884)

抗原

See all CBL 抗体

CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This CBL antibody is conjugated to PE

应用范围

Intracellular Staining (ICS)

克隆位点

47-c

Discover our top product CBL Primary Antibody

抗原表位
25

22

19

17

9

6

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr700

品牌

BD Phosflow™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human c-Cbl (pY700) Peptide

亚型

IgG1
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody
CBL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3B12 unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 736-835) antibody
CBL 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 6D12 unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 1-906) antibody
CBL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 1-906) antibody
CBL 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (Internal Region) antibody
CBL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 548-847) antibody
CBL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr770) antibody
CBL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody
CBL 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr674) antibody
CBL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 637-906) antibody
CBL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

样本量

20 μL

限制

仅限研究用
状态

Liquid

缓冲液

Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Miura-Shimura, Duan, Rao, Reddi, Shimura, Rottapel, Druker, Tsygankov, Band, Band: "Cbl-mediated ubiquitinylation and negative regulation of Vav." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38495-504, (2003) (PubMed).

Thien, Langdon: "Cbl: many adaptations to regulate protein tyrosine kinases." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 294-307, (2001) (PubMed).

Tsygankov, Teckchandani, Feshchenko, Swaminathan: "Beyond the RING: CBL proteins as multivalent adapters." in: Oncogene, Vol. 20, Issue 44, pp. 6382-402, (2001) (PubMed).
抗原

CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

别名

c-Cbl

背景

Cbl (Casitas B-lineage lymphoma) was identified in the genome of a transforming retrovirus from a mouse pre-B lymphoma. The cellular gene product c-Cbl is one of numerous Cbl-related proteins found in vertebrate and invertebrate organisms. It is an 120-kDa adapter protein that contains multiple functional domains, including a RING finger motif, a tyrosine kinase-binding (TKB) domain, and a proline-rich region. The TKB domain directly interacts with specific auto-phosphorylation sites in activated protein-tyrosine kinases (PTK). Through the RING finger motif, c-Cbl recruits and activates an E2 ubiquitin-conjugating enzyme, thus targeting the activated PTK for protein degradation. The proline-rich region contains SH3 domain-binding and 14-3-3 protein-binding motifs. c-Cbl is also phosphorylated at tyrosines 700 (Y700), 731, and 774 by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for antigens, immunoglobulins, growth factors, cytokines, and hormones. In turn, the phosphorylated Y700 site interacts with the SH2 domains of CRK and Vav1. The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells. Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. The 47/c-Cbl monoclonal antibody recognizes the Y700-phosphorylated form of human c-Cbl. The orthologous phosphorylation site in mouse c-Cbl is Y698.

途径

TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals