(3 references)-
- 抗原 See all CBL 抗体
- CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))
-
抗原表位
- pTyr700
-
适用
- 人, 小鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This CBL antibody is conjugated to PE
-
应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human c-Cbl (pY700) Peptide
- 克隆位点
- 47-c
- 亚型
- IgG1
- Top Product
- Discover our top product CBL Primary Antibody
-
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody
CBL 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3B12 unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 736-835) antibodyCBL 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 6D12 unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 1-906) antibodyCBL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 1-906) antibodyCBL 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (Internal Region) antibodyCBL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 548-847) antibodyCBL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr770) antibodyCBL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibodyCBL 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (pTyr674) antibodyCBL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) (AA 637-906) antibodyCBL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
- 样本量
- 20 μL
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
-
-
: "Cbl-mediated ubiquitinylation and negative regulation of Vav." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38495-504, (2003) (PubMed).
: "Cbl: many adaptations to regulate protein tyrosine kinases." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 294-307, (2001) (PubMed).
: "Beyond the RING: CBL proteins as multivalent adapters." in: Oncogene, Vol. 20, Issue 44, pp. 6382-402, (2001) (PubMed).
-
: "Cbl-mediated ubiquitinylation and negative regulation of Vav." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38495-504, (2003) (PubMed).
-
- 抗原
- CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))
- 别名
- c-Cbl (CBL 产品)
- 背景
- Cbl (Casitas B-lineage lymphoma) was identified in the genome of a transforming retrovirus from a mouse pre-B lymphoma. The cellular gene product c-Cbl is one of numerous Cbl-related proteins found in vertebrate and invertebrate organisms. It is an 120-kDa adapter protein that contains multiple functional domains, including a RING finger motif, a tyrosine kinase-binding (TKB) domain, and a proline-rich region. The TKB domain directly interacts with specific auto-phosphorylation sites in activated protein-tyrosine kinases (PTK). Through the RING finger motif, c-Cbl recruits and activates an E2 ubiquitin-conjugating enzyme, thus targeting the activated PTK for protein degradation. The proline-rich region contains SH3 domain-binding and 14-3-3 protein-binding motifs. c-Cbl is also phosphorylated at tyrosines 700 (Y700), 731, and 774 by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for antigens, immunoglobulins, growth factors, cytokines, and hormones. In turn, the phosphorylated Y700 site interacts with the SH2 domains of CRK and Vav1. The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells. Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. The 47/c-Cbl monoclonal antibody recognizes the Y700-phosphorylated form of human c-Cbl. The orthologous phosphorylation site in mouse c-Cbl is Y698.
- 途径
- TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals
-
You are here:
