CCL2 抗体
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北京 101111
Quick Overview for CCL2 抗体 (ABIN1176870)
抗原
See all CCL2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse MCP-1. Neutralization activity is measured in a calcium flux assay using 100-200 ng/mL recombinant mouse MCP-1 (Cat. No. 554590) to stimulate THP-1 indicator cells.
50% Neutralization (ND50) at 2 - 30 myg/mL
90% Neutralization at 40 - 300 myg/mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Heparin-purified CHO-expressed mouse MCP-1
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亚型
- IgG1 kappa
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anti-Chemokine (C-C Motif) Ligand 2 (CCL2) antibody
CCL2 适用: 人, 小鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 61-148) antibodyCCL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) antibodyCCL2 适用: 人 WB, IHC, ELISA, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-96) antibodyCCL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 62-89), (C-Term) antibodyCCL2 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB17838 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 62-89) antibodyCCL2 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) antibodyCCL2 适用: 人, 小鼠, 猴 WB, IHC (p), IF, FACS, EIA 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) antibodyCCL2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) antibodyCCL2 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) antibodyCCL2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: The purified format of the 2H5 antibody (Cat. No. 551217) is useful as a capture antibody in a sandwich ELISA for measuring mouse MCP-1 protein levels when paired with biotin conjugated clone 4E2/MCP (Cat. No, 554444) as a detection reagent and recombinant mouse MCP-1 (Cat. No. 554590) as the standard. For testing mouse MCP-1 in complex biological fluids, such as serum or plasma, investigators are encouraged to consider using the BD OptEIA™ Mouse MCP-1 Set (Cat. No. 555260).
Flow Cytometry: The 2H5 antibody can be useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate MCP-1 producing cells within mixed cell populations. The PE-conjugated 2H5 antibody (Cat. No. 554443) is especially suitable for these experiments. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3708-16, (1994) (PubMed).
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
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- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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别名
- MCP-1
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背景
- The 2H5 antibody reacts with mouse and rat monocyte chemoattractant protein (MCP-1), formerly termed JE. This antibody also recognizes human MCP-1, but shows no reactivity with the closely related mouse beta chemokines, TCA3 and MIP-1beta. The immunogen used to generate the 2H5 hybridoma was heparin-purified CHO-expressed mouse MCP-1. This is a neutralizing antibody.
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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