HMGB1 抗体
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北京 101111
Quick Overview for HMGB1 抗体 (ABIN1176834)
抗原
See all HMGB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti HMGB1 monoclonal antibody
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序列
- KGKPDAAKKGVVKAEKS
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交叉反应 （详细）
- DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2.
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产品特性
- DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.
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免疫原
- The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide P1 (KGKPDAAKKGVVKAEKS). Hybridomas were generated from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.
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亚型
- IgG1
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anti-High Mobility Group Box 1 (HMGB1) (AA 75-170) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) antibodyKO Validated HMGB1 适用: 人 WB, IHC, IF, ICC, IHC (p), IHC (wm), IHC (ff) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-90) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 9-163) antibodyHMGB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E6-E10 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 124-154), (C-Term) antibodyHMGB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
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应用备注
- WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.
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说明
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DPH1.1 mAb can be used for Western blot (WB).
DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays.
In vivo, DPH1.1 mAb administered intravenously (220 μg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The lyophilized protein once reconstituted will be dissolved in a solution containing Na3PO4 50mM and NaCl 0.15M pH 7.4.
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缓冲液
- The antibody is lyophilized from Na3PO4 50mM and NaCl 0.15M pH 7.4
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储存条件
- -20 °C
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储存方法
- 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C), thawed and re-frozen.
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: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." in: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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别名
- High-mobility group protein B1 (HMGB1)
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背景
- HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.
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途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
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