HMGB1 抗体
(1 reference)Quick Overview for HMGB1 抗体 (ABIN1176834)
抗原
See all HMGB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- 17-mer peptide KGKPDAAKKGVVKAEKS
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亚型
- IgG1
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anti-High Mobility Group Box 1 (HMGB1) (AA 75-170) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) antibodyKO Validated HMGB1 适用: 人 WB, IHC, IF, ICC, IHC (p), IHC (wm), IHC (ff) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-90) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 9-163) antibodyHMGB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E6-E10 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 124-154), (C-Term) antibodyHMGB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
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应用备注
- WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.
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限制
- 仅限研究用
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状态
- Lyophilized
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注意事项
- DPH1.1 mAb is provided freeze-dried.
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储存条件
- -20 °C
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: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." in: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).
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: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." in: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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别名
- High-mobility group protein B1 (HMGB1)
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背景
- DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.
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途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
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