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APOB 抗体 (AA 3329-3494) (Biotin)

APOB 适用: 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN1175346
发货至: 中国
  • 抗原 See all APOB 抗体
    APOB (Apolipoprotein B (APOB))
    抗原表位
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 3329-3494
    适用
    • 91
    • 25
    • 21
    • 6
    • 3
    大鼠
    宿主
    • 61
    • 34
    • 15
    • 2
    • 1
    克隆类型
    • 77
    • 34
    多克隆
    标记
    • 47
    • 20
    • 17
    • 7
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This APOB antibody is conjugated to Biotin
    应用范围
    • 64
    • 45
    • 32
    • 17
    • 15
    • 14
    • 10
    • 7
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    序列
    MGHHHHHHSG S-FL SSSSFVTDAL QYKLEGTSRL MRKKVLKLAT AVSLTNKFLK GSHDSTISLT KKNMEASVKT TANLHAPIFT MNFKQELNGN TKSKPTVSSS IELNYDFNSS KLHSAAKGGV DHKFSLESLT SYLSIESFTK GNIKGSFLSQ EYSGSVANEA NVYLNSKGTR SSVR
    特异性
    It has been selected for its ability to recognize APOB in immunohistochemical staining and Western blotting.
    纯化方法
    Affinity Chromatography
    免疫原
    Recombinant APOB expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against APOB conjugated to biotin.
    亚型
    IgG
    Top Product
    Discover our top product APOB Primary Antibody
  • 应用备注
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    说明

    Content: The quality control contains recombinant APOB (Phe3329~Arg3494) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freeze/thaw cycles
    储存条件
    4 °C
    储存方法
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    有效期
    12 months
  • 抗原
    APOB (Apolipoprotein B (APOB))
    Abstract
    APOB 产品
    别名
    FLDB antibody, LDLCQ4 antibody, Ac1-060 antibody, Apo B-100 antibody, ApoB-100 antibody, ApoB-48 antibody, AI315052 antibody, apob-100 antibody, apob-48 antibody, APOB-100 antibody, ApoB(100) antibody, APOB antibody, apolipoprotein B antibody, APOB antibody, Apob antibody
    途径
    Lipid Metabolism
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